EFFICIENCY OF USING ALAMARBLUE CELL VIABILITY REAGENT TO STUDY EFFECTS OF CARBON NANOPARTICLES ON CANCER CELLS

Abstract

Abstract. Background: Nanoparticles have been widely used to deliver drugs, image tumors inhibit cancer cells, and to apply in other diseases. In cancer treatment, we usually need t o test viability on cell lines in vitro. Cell counting or viability testing reagents have been applied. However, special characteristics of nanoparticles such as color, fluorescence, and organelle binding may interfere the dye binding. Here we studied the effects of alamarBlue Cell Viability reagent staining conditions on cell viability measurement efficiency.